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Agric Biol Chem. 1990 Jun;54(6):1453-7.

Cloning and expression of the creatinase gene from Flavobacterium sp. U-188 in Escherichia coli.

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Research and Development Division, Kikkoman Corporation, Chiba, Japan.


The gene coding for creatinase (creatine amidinohydrolase, EC was isolated from Flavobacterium sp. U-188. The primary structure of creatinase deduced from the nucleotide sequence showed a protein (molecular weight, 42, 651) composed of 378 amino acids. The creatinase gene was over-expressed in Escherichia coli under the control of the lac promoter and the amount of this enzyme was over 20% of the soluble protein in the cell.

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