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Biosci Biotechnol Biochem. 1992 Jun;56(6):906-12.

Cloning and sequencing of the xynA gene encoding xylanase A of Aspergillus kawachii.

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1
National Research Institute of Brewing, Tokyo, Japan.

Abstract

We have cloned the xynA gene coding for xylanase A, a major component of the xylanase family, from Aspergillus kawachii. The cDNA was isolated from an A. kawachii cDNA library by immunoscreening using antibody raised against the purified xylanase A protein. Nucleotide sequence analysis of the cDNA showed a 981-bp open reading frame that encoded a protein of 327 amino acid residues. The signal peptide was composed of 25 amino acid residues and the N-terminus of the mature protein was pyroglutamic acid. The transformed yeast with a cloned cDNA produced xylanase. The genomic DNA was arranged as ten exons and nine introns.

PMID:
1368254
[Indexed for MEDLINE]
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