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Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11350-5. Epub 2003 Sep 17.

Single metallic nanoparticle imaging for protein detection in cells.

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1
Centre de Physique Moléculaire Optique et Hertzienne, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5798 et Université Bordeaux 1, 351 Cours de la Libération, 33405 Talence, France.

Abstract

We performed a visualization of membrane proteins labeled with 10-nm gold nanoparticles in cells, using an all-optical method based on photothermal interference contrast. The high sensitivity of the method and the stability of the signals allows 3D imaging of individual nanoparticles without the drawbacks of photobleaching and blinking inherent to fluorescent markers. A simple analytical model is derived to account for the measurements of the signal amplitude and the spatial resolution. The photothermal interference contrast method provides an efficient, reproducible, and promising way to visualize low amounts of proteins in cells by optical means.

PMID:
13679586
PMCID:
PMC208760
DOI:
10.1073/pnas.1534635100
[Indexed for MEDLINE]
Free PMC Article
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