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Appl Microbiol Biotechnol. 1990 Sep;33(6):680-2.

Cloning of a gene coding for phosphotransacetylase from Escherichia coli.

Author information

1
Research and Development Division, Kikkoman Corporation, Chiba, Japan.

Abstract

A phosphotransacetylase gene (pta) has been cloned from a genomic DNA library of Escherichia coli 1100, a derivative of strain K-12. The phosphotransacetylase activities of pta+ plasmid-containing strains were amplified about 150-fold under control of the lac promoter. The molecular weight of the phosphotransacetylase was estimated to be about 81,000 by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The pta gene was found to be downstream of ackA by a combination of restriction analysis and plasmid subcloning. It is located about 13 kb upstream of the purF-folC-hisT region of the chromosome.

PMID:
1366944
DOI:
10.1007/bf00604937
[Indexed for MEDLINE]

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