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J Biotechnol. 1990 Apr;14(1):43-52.

Determination of alanine aminotransferase in human serum in an open-closed flow injection configuration.

Author information

1
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.

Abstract

Open-closed flow injection systems allow the development of methods based on multidetection by using conventional detectors. The methods proposed here for the determination of alanine aminotransferase (ALT) utilize a photometric detector included in the closed circuit, which allows one to perform fixed-time and reaction-rate measurements whose sensitivity can be increased by using the sum of the analytical signals. The methods, with determination ranges between 1 and 500 U l-1 of ALT, feature sampling frequencies up to 60 h-1. Their application to serum samples provided excellent results, with recoveries between 95 and 106% and averaging at 99.9%.

PMID:
1366447
DOI:
10.1016/0168-1656(90)90016-5
[Indexed for MEDLINE]

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