Recombinant polypeptide from the gp48 region of the bovine viral diarrhea virus (BVDV) detects serum antibodies in vaccinated and infected cattle

Vet Microbiol. 1992 Oct;32(3-4):281-92. doi: 10.1016/0378-1135(92)90151-i.

Abstract

To characterize the immune response of cattle to bovine viral diarrhea virus (BVDV) glycoprotein gp48, we have produced a large amount of recombinant glutathione-s-transferase-gp48 (GST-gp48) fusion protein in Escherichia coli. Antibodies to gp48 were present in cattle vaccinated with killed or modified-live virus vaccination, or following natural infection. These results were in agreement with results of serum neutralization (SN) test which detected gp53 of BVDV.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Viral / blood*
  • Base Sequence
  • Blotting, Western
  • Bovine Virus Diarrhea-Mucosal Disease / immunology*
  • Cattle
  • Diarrhea Viruses, Bovine Viral / immunology*
  • Gene Expression Regulation, Viral
  • Genetic Vectors
  • Glutathione Transferase / chemistry
  • Glycoproteins / chemistry
  • Glycoproteins / immunology*
  • Molecular Sequence Data
  • Neutralization Tests
  • Recombinant Fusion Proteins* / chemistry
  • Vaccination / veterinary
  • Viral Envelope Proteins / chemistry
  • Viral Envelope Proteins / immunology*
  • Viral Vaccines / immunology*

Substances

  • Antibodies, Viral
  • Glycoproteins
  • Recombinant Fusion Proteins
  • Viral Envelope Proteins
  • Viral Vaccines
  • glycoprotein 48, bovine viral diarrhea virus
  • Glutathione Transferase