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Vet Microbiol. 1992 Oct;32(3-4):253-65.

Foot-and-mouth disease: detection of antibodies in cattle sera by blocking ELISA.

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Veterinary Research Laboratory, Harare, Zimbabwe.


A blocking ELISA was developed for the detection of antibodies to foot-and-mouth disease virus SAT1, SAT2 and SAT3 and for the quantification of antibodies on a single dilution of serum. The avidin-biotin system was used. The test was compared with the liquid-phase ELISA executed at the World Reference Laboratory for foot-and-mouth disease. It was found to have favourable logistics and combined high specificity with high sensitivity. The quantitative test using a single dilution of serum was resource saving and proved to be a reliable and precise method for the assessment of antibody levels.

[Indexed for MEDLINE]

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