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Arthritis Rheum. 1992 Oct;35(10):1179-84.

Light microscopic characterization of the fibroblast-like synovial intimal cell (synoviocyte).

Author information

1
Department of Rheumatology Research, University College and Middlesex School of Medicine, London, England.

Abstract

OBJECTIVE:

To reassess synovial intimal cell populations by light microscopy.

METHODS:

Non-inflamed, rheumatoid and osteoarthritic synovia were analyzed as tissue sections and cytospin preparations by a series of combined immunohistochemical and cytochemical staining techniques.

RESULTS:

Two populations of intimal cells were identified. The first carried macrophage markers. The second showed high uridine diphosphoglucose dehydrogenase (UDPGD) activity, minimal cytoplasmic CD68, absent non-specific esterase (NSE) activity, and absent leukocyte and endothelial antigens. The majority of these cells showed a high content of prolyl hydroxylase.

CONCLUSION:

Combined cytochemical staining for NSE and UDPGD activity allows effective separation of intimal cell populations. We suggest that the cells of high UDPGD activity are the fibroblast-like or type B synovial intimal cells defined by electron microscopy. High UDPGD activity probably reflects a preferential ability to synthesize glycosaminoglycans, including hyaluronan.

PMID:
1329776
DOI:
10.1002/art.1780351010
[Indexed for MEDLINE]

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