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Jpn J Cancer Res. 1992 Aug;83(8):795-7.

Preparation and characterization of a murine monoclonal antibody (MDR3M) reactive with mdr3 gene product.

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1
Department of Pathology, Saitama Medical Center, Saitama Medical School.

Abstract

A murine monoclonal antibody (MDR3M) (isotype: IgM) reactive with mdr3 gene product was generated by immunizing mice with mdr3-specific peptide (H2N-12WRPTSAEGDFELGISSKQKRKKTKTVKMI41G-COOH) and hybridizing the primed mouse splenic B cells with X63-Ag8,6.5.3 mouse plasmacytoma cells. MDR3M did not cross-react with mdr1 gene product. This monoclonal antibody may be useful for analyzing the role of mdr3 gene product in cells and tissues.

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