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Epidemiol Infect. 1992 Oct;109(2):303-12.

Identification of human and bovine rotavirus serotypes by polymerase chain reaction.

Author information

1
Department of Hygiene, Sapporo Medical College, Japan.

Abstract

The use of the polymerase chain reaction (PCR) for identifying serotypes of human and bovine rotaviruses was examined. In the identification of 115 human rotavirus samples in stools, results with PCR showed excellent agreement with results of serotyping by an enzyme-linked immunosorbent assay (ELISA) using serotype-specific monoclonal antibodies. Furthermore, the PCR showed a much higher sensitivity (93%) than the ELISA test (82.6%). The PCR method could also be applied for identifying the serotype of bovine rotaviruses.

PMID:
1327857
PMCID:
PMC2271918
DOI:
10.1017/s0950268800050263
[Indexed for MEDLINE]
Free PMC Article

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