Abstract
We investigated the mechanism by which cyclic AMP (cAMP) induces gap junctional communication via cell-to-cell channels in a communication-deficient rat Morris hepatoma cell line. We found that under basal conditions, the cells transcribe cx43 at a low level but do not transcribe cx26 or cx32. Elevation of intracellular cAMP, which induced communication, increased cx43 mRNA 15- to 40-fold and the rate of cx43 transcription 6-fold. Cx43 protein was detected by immunostaining in junctions of only those cells in which communication had been induced. We found the regulation by cAMP also in other cell lines; namely, in those with a low basal level of cx43 mRNA.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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8-Bromo Cyclic Adenosine Monophosphate / pharmacology
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Animals
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Blotting, Northern
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Cell Communication
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Cell Division / drug effects
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Cell Division / physiology
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Cell Line
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Cell Line, Transformed
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Colforsin / pharmacology
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Connexins
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Cyclic AMP / physiology*
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DNA Probes
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Gene Expression Regulation*
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Immunoblotting
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Intercellular Junctions / physiology*
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Liver Neoplasms, Experimental
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Membrane Proteins / genetics*
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Protein Biosynthesis
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RNA, Messenger / genetics
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Rats
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Transcription, Genetic
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Tumor Cells, Cultured
Substances
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Connexins
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DNA Probes
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Membrane Proteins
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RNA, Messenger
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Colforsin
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8-Bromo Cyclic Adenosine Monophosphate
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Cyclic AMP