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Mol Plant Microbe Interact. 1992 Jul-Aug;5(4):330-9.

Cloning and heterologous expression of the phenazine biosynthetic locus from Pseudomonas aureofaciens 30-84.

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1
Agricultural Research Service, U.S. Department of Agriculture, Washington State University, Pullman 99164-6430.

Abstract

Pseudomonas aureofaciens strain 30-84 suppresses take-all disease of wheat caused by Gaeumannomyces graminis var. tritici. Three antibiotics, phenazine-1-carboxylic acid, 2-hydroxyphenazine-1-carboxylic acid, and 2-hydroxyphenazine, were responsible for disease suppression. Tn5-induced mutants deficient in production of one or more of the antibiotics (Phz-) were significantly less suppressive than the parental strain. Cosmids pLSP259 and pLSP282 from a genomic library of strain 30-84 restored phenazine production and fungal inhibition to 10 different Phz- mutants. Sequences required for production of the phenazines were localized to a segment of approximately 2.8 kilobases that was present in both cosmids. Expression of this locus in Escherichia coli required the introduction of a functional promoter, was orientation-specific, and resulted in the production of all three phenazine antibiotics. These results strongly suggest that the cloned sequences encode a major portion of the phenazine biosynthetic pathway.

PMID:
1325219
DOI:
10.1094/mpmi-5-330
[Indexed for MEDLINE]

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