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Trends Biochem Sci. 1992 Jul;17(7):246-9.

Blotting and band-shifting: techniques for studying protein-protein interactions.

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Vollum Institute for Advanced Biomedical Research, Portland, OR 97201-3089.


The type II cAMP-dependent protein kinase (PKA) is localized in certain cellular compartments through association with specific A-kinase anchoring proteins (AKAPs). A variety of blotting and electrophoresis techniques have been developed to study the protein-protein interactions that occur between the regulatory (R) subunit of PKA and AKAPs. These methods have also been used for a variety of purposes such as detecting calmodulin-binding proteins, comparing wild-type- and mutant-form binding affinities and estimating the molecular weight of multiprotein complexes.

[Indexed for MEDLINE]

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