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J Mol Evol. 1992 May;34(5):379-82.

Calculation of sequence divergence from the thermal stability of DNA heteroduplexes.

Author information

1
Kerckhoff Marine Laboratory, California Institute of Technology, Corona del Mar 92625.

Abstract

Measurements are reported of the thermal stability of DNA heteroduplexes between clones of the eta-globin pseudogene from a variety of primates. The known sequences of this 7.1-kb region differ from each over a range from 1.6% for human versus chimp to nearly 12% for human versus spider monkey. Thermal stability was determined by standard hydroxyapatite thermal elution, and the results show a precisely linear decrease in thermal stability with divergence. The slope of the regression line is 1.18% sequence divergence per degree centigrade reduction in thermal stability.

PMID:
1318388
[Indexed for MEDLINE]

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