Send to

Choose Destination
J Mol Evol. 1992 May;34(5):379-82.

Calculation of sequence divergence from the thermal stability of DNA heteroduplexes.

Author information

Kerckhoff Marine Laboratory, California Institute of Technology, Corona del Mar 92625.


Measurements are reported of the thermal stability of DNA heteroduplexes between clones of the eta-globin pseudogene from a variety of primates. The known sequences of this 7.1-kb region differ from each over a range from 1.6% for human versus chimp to nearly 12% for human versus spider monkey. Thermal stability was determined by standard hydroxyapatite thermal elution, and the results show a precisely linear decrease in thermal stability with divergence. The slope of the regression line is 1.18% sequence divergence per degree centigrade reduction in thermal stability.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center