Abstract
1. The existence of both cAMP-responsive element binding factor and a nuclear factor 1-like (NF-1-like) protein in nuclear extracts from liver of cAMP-treated rat has been revealed. 2. Binding of these proteins to a DNA fragment containing both elements was cooperative, and 50% binding was achieved with considerably less protein than with a fragment bearing either element alone. 3. Cleavage of the fragment between the two elements abolished the apparent cooperative interaction. 4. Southwestern blot analysis showed that the NF-1-like protein has a molecular weight in the 28-30-kDa range. 5. The NF-1-like binding activity was very stable.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Blotting, Southern
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Blotting, Western
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CCAAT-Enhancer-Binding Proteins*
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Cell Nucleus / chemistry
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Cyclic AMP / pharmacology
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Cyclic AMP Response Element-Binding Protein
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DNA / chemistry
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DNA / metabolism*
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DNA-Binding Proteins / metabolism*
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Liver / chemistry*
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Liver / drug effects
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Molecular Sequence Data
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NFI Transcription Factors
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Nuclear Proteins
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Phosphoenolpyruvate Carboxykinase (GTP) / genetics*
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Promoter Regions, Genetic
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Rats
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Regulatory Sequences, Nucleic Acid
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Time Factors
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Transcription Factors*
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Y-Box-Binding Protein 1
Substances
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CCAAT-Enhancer-Binding Proteins
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Cyclic AMP Response Element-Binding Protein
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DNA-Binding Proteins
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NFI Transcription Factors
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Nuclear Proteins
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Transcription Factors
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Y-Box-Binding Protein 1
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YBX1 protein, human
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DNA
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Cyclic AMP
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Phosphoenolpyruvate Carboxykinase (GTP)