Interaction of a nuclear factor 1-like protein with a cAMP response element-binding protein in rat liver

Int J Biochem. 1992 Mar;24(3):455-64. doi: 10.1016/0020-711x(92)90039-4.

Abstract

1. The existence of both cAMP-responsive element binding factor and a nuclear factor 1-like (NF-1-like) protein in nuclear extracts from liver of cAMP-treated rat has been revealed. 2. Binding of these proteins to a DNA fragment containing both elements was cooperative, and 50% binding was achieved with considerably less protein than with a fragment bearing either element alone. 3. Cleavage of the fragment between the two elements abolished the apparent cooperative interaction. 4. Southwestern blot analysis showed that the NF-1-like protein has a molecular weight in the 28-30-kDa range. 5. The NF-1-like binding activity was very stable.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Southern
  • Blotting, Western
  • CCAAT-Enhancer-Binding Proteins*
  • Cell Nucleus / chemistry
  • Cyclic AMP / pharmacology
  • Cyclic AMP Response Element-Binding Protein
  • DNA / chemistry
  • DNA / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Liver / chemistry*
  • Liver / drug effects
  • Molecular Sequence Data
  • NFI Transcription Factors
  • Nuclear Proteins
  • Phosphoenolpyruvate Carboxykinase (GTP) / genetics*
  • Promoter Regions, Genetic
  • Rats
  • Regulatory Sequences, Nucleic Acid
  • Time Factors
  • Transcription Factors*
  • Y-Box-Binding Protein 1

Substances

  • CCAAT-Enhancer-Binding Proteins
  • Cyclic AMP Response Element-Binding Protein
  • DNA-Binding Proteins
  • NFI Transcription Factors
  • Nuclear Proteins
  • Transcription Factors
  • Y-Box-Binding Protein 1
  • YBX1 protein, human
  • DNA
  • Cyclic AMP
  • Phosphoenolpyruvate Carboxykinase (GTP)