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Nature. 1992 Jan 16;355(6357):224-30.

Mapping T-cell receptor-peptide contacts by variant peptide immunization of single-chain transgenics.

Author information

1
Department of Microbiology and Immunology, Stanford University, California 94305.

Abstract

To test models of T-cell recognition, mice transgenic for T-cell receptor alpha or beta chain have been immunized with variant peptides that force changes in the resulting T-cell response. In particular, charge substitutions on the peptide often elicit reciprocal charges in the junctional (CDR3) sequences of T-cell receptor V alpha or V beta chains, indicating direct T-cell receptor-peptide contact, and allowing derivation of a topology for the T-cell receptor-MHC interaction. At one position on the peptide, variants transformed a homogeneous V beta response into a very heterogeneous one.

PMID:
1309938
DOI:
10.1038/355224a0
[Indexed for MEDLINE]

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