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Mol Mar Biol Biotechnol. 1992 Apr;1(2):136-46.

Automated sequential affinity chromatography of sea urchin embryo DNA binding proteins.

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Division of Biology, California Institute of Technology, Pasadena 91125.


An automated method of running a tandem sequence of oligonucleotide affinity columns was used to purify factors that interact specifically with cis-regulatory sites of the CyIIIa cytoskeletal actin gene of the sea urchin embryo (Strongylocentrotus purpuratus). The method allows quantitative enrichment in a single chromatographic run of up to 12 different sequence-specific DNA binding proteins, each of which may then be readily purified to homogeneity by methods such as preparative gel electrophoresis. The affinity chromatography and identification of six different CyIIIa-regulatory factors is described, and the general utility of the method is discussed.

[Indexed for MEDLINE]

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