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Neurochem Int. 1992 Oct;21(3):409-14.

GTP-binding proteins in bovine brain nuclear membranes.

Author information

1
Arbeitsgruppe Neurochemie, Institut für Biochemie, Freie Universität Berlin, Germany.

Abstract

Nuclear membranes and other subcellular fractions derived from bovine brain cortex were investigated for the existence of GTP-binding proteins. By using photolytic labeling with [alpha-32P]GTP a 29 kDa GTP-binding protein was shown to be present in nuclear membranes which was not present in the plasma membranes nor in microsomal or cytosolic fractions. Two-dimensional gel electrophoresis revealed that this protein is rather acidic with a pI lower than 4.5. Members of the heterotrimeric Gi/o family are not present in the nuclear envelope: a 39 kDa protein, ADP ribosylated by pertussis toxin, was shown to originate from plasma membrane contamination.

PMID:
1303165
DOI:
10.1016/0197-0186(92)90192-t
[Indexed for MEDLINE]

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