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[Analysis on quality control of blood in blood banks in a five-year program in Hunan province].

[Article in Chinese]

Author information

1
Hunan Center for Clinical Laboratory, Changsha 410008, China.

Abstract

OBJECTIVE:

To investigate the blood quality of blood banks.

METHODS:

HBsAg, Anti-HCV, Anti-HIV were detected by enzyme linked immunoadsorbent assay (ELISA). Treponema pallidum was detected by Trust or rapid plasma regain card test (RPR) before August 2001 and ELISA was used afterwards. Alanine aminotransferase (ALT) was detected by Reitman-Frankel before December 1999 and by continuous monitoring assay afterwards. Results were compared between (1) sampling and spot check, (2) different determent methods, (3) two kinds of reagents with different antigen coating.

RESULTS:

Seventy-two (8.28 per thousand ) of the 8,699 plasma samples were found unqualified with levels of anti-HCV, ALT, HBsAg, Treponema pallidum and anti-HIV 24 (2.76 per thousand ), 19 (2.18 per thousand ), 16 (1.84 per thousand ), 7 (0.80 per thousand ), 6 (0.69 per thousand ), respectively. There were significant differences between different determent methods and different reagents, but only ALT showed significant difference in the sampling and spot check.

CONCLUSION:

The unqualified samples were associated with testing methods, quality of reagents as well as ability and responsibility of the staff.

PMID:
12975011
[Indexed for MEDLINE]

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