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Appl Environ Microbiol. 2003 Sep;69(9):5726-30.

Molecular detection and quantitation of the red tide dinoflagellate Karenia brevis in the marine environment.

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1
University of South Florida, College of Marine Science, St. Petersburg, Florida 33701, USA.

Abstract

A real-time reverse transcription-PCR method targeting the rbcL gene was developed for the detection and quantitation of the Florida red tide organism, Karenia brevis. The assay was sensitive to less than 1 cell per reaction, did not detect rbcL from 38 nontarget taxa, and accurately quantitated K. brevis organisms in red tide samples from around Florida. These studies have resulted in a sensitive and specific method for K. brevis detection in the marine environment.

PMID:
12957971
PMCID:
PMC194946
[Indexed for MEDLINE]
Free PMC Article
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