Send to

Choose Destination
J Immunol Methods. 2003 Jul;278(1-2):283-92.

Simple and cost-effective isolation of monocytes from buffy coats.

Author information

Blood Transfusion Centre of Slovenia, Tissue Typing Centre, Slajmerjeva 6, 1000 Ljubljana, Slovenia.


We have optimized the procedure of monocyte isolation on a Percoll density gradient. The new procedure consists of three steps: (1) the isolation of MNC on a Ficoll density gradient; (2) the separation of monocytes from lymphocytes on a high-density hyper-osmotic Percoll density gradient; and (3) the separation of monocytes from platelets and dead cells on a low-density iso-osmotic Percoll density gradient. The procedure is simple and cost-effective. Monocyte purity and recovery are both about 75% and platelet contamination is low. The isolated monocytes retain their capacity to differentiate into dendritic cells in vitro.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center