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J Immunol Methods. 2003 Jul;278(1-2):283-92.

Simple and cost-effective isolation of monocytes from buffy coats.

Author information

1
Blood Transfusion Centre of Slovenia, Tissue Typing Centre, Slajmerjeva 6, 1000 Ljubljana, Slovenia. urska.repnik@guest.arnes.si

Abstract

We have optimized the procedure of monocyte isolation on a Percoll density gradient. The new procedure consists of three steps: (1) the isolation of MNC on a Ficoll density gradient; (2) the separation of monocytes from lymphocytes on a high-density hyper-osmotic Percoll density gradient; and (3) the separation of monocytes from platelets and dead cells on a low-density iso-osmotic Percoll density gradient. The procedure is simple and cost-effective. Monocyte purity and recovery are both about 75% and platelet contamination is low. The isolated monocytes retain their capacity to differentiate into dendritic cells in vitro.

PMID:
12957415
DOI:
10.1016/s0022-1759(03)00231-x
[Indexed for MEDLINE]

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