Abstract
The bacterial version of the mammalian signal recognition particle (SRP) is well conserved and essential to all known bacteria. The genes for the Streptomyces lividans SRP components have been cloned and characterized. FtsY resembles the mammalian SRP receptor and the S. lividans SRP consists of Ffh, a homologue of the mammalian SRP54 protein, and scRNA, which is a small size RNA of 82 nt in length. Co-immunoprecipitation studies confirmed that Ffh and scRNA are probably the only components of the S. lividans SRP and that pre-agarase can co-immunoprecipitate with Ffh, suggesting that the SRP is involved in targeting secretory proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Genome, Bacterial
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Molecular Sequence Data
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Precipitin Tests
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RNA, Small Cytoplasmic
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / metabolism*
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Receptors, Cytoplasmic and Nuclear / metabolism
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Receptors, Peptide / metabolism
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Recombinant Fusion Proteins / metabolism
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Signal Recognition Particle / chemistry
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Signal Recognition Particle / genetics
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Signal Recognition Particle / metabolism*
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Streptomyces / genetics
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Streptomyces / metabolism*
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Transcription, Genetic
Substances
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Bacterial Proteins
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FtsY protein, Bacteria
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RNA, Small Cytoplasmic
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RNA-Binding Proteins
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Receptors, Cytoplasmic and Nuclear
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Receptors, Peptide
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Recombinant Fusion Proteins
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Signal Recognition Particle
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signal peptide receptor
Associated data
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GENBANK/AF071565
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GENBANK/AY081854
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GENBANK/AY140960