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J Virol. 2003 Sep;77(18):10119-24.

Analysis of early human immunodeficiency virus type 1 DNA synthesis by use of a new sensitive assay for quantifying integrated provirus.

Author information

1
Département des Maladies Infectieuses, Institut Cochin, INSERM U567, CNRS UMR 8104, Université René Descartes, 22 rue Méchain, 75014 Paris, France.

Abstract

A novel Alu-long terminal repeat (LTR)-based real-time nested-PCR assay was developed to quantify integrated human immunodeficiency virus type 1 (HIV-1) DNA in infected cells with both accuracy and high sensitivity (six proviruses within 50,000 cell equivalents). Parallel assays for total HIV-1 DNA and two-LTR HIV-1 DNA circles allowed the synthesis and fate of the different HIV-1 DNA species to be monitored upon a single round of viral replication.

PMID:
12941923
PMCID:
PMC224570
DOI:
10.1128/jvi.77.18.10119-10124.2003
[Indexed for MEDLINE]
Free PMC Article

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