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FEBS Lett. 2003 Aug 28;550(1-3):155-62.

A novel role for proliferin-2 in the ex vivo expansion of hematopoietic stem cells.

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  • 1Bioprocessing Technology Centre, MD 11 Level 5, 10 Medical Drive, Singapore 117597. btccml@btc.a-star.edu.sg

Abstract

A family of proliferin genes was discovered on a microarray analysis of hematopoiesis supportive stromal cell lines. Proliferin-2 (PLF2) increased the frequency of long-term culture-initiating cells (LTC-IC) from 1 in 340 to 1 in 256 of the primary hematopoietic stem cell (HSC)-enriched bone marrow cells grown on MS5.1 feeder layer. A repeat using AFT024 feeder layer also showed a similar increase in LTC-IC (from 1 in 386 cells to 1 in 260 cells). The clonogenic output of the LTC-ICs was also increased significantly. The growth of various hematopoietic and stromal cell lines treated with PLF2 was found to increase by 4-27%, as measured by cell count and DNA synthesis assay. These findings open up the possibility of using PLF2 as a new member of the growth factor cocktails for the ex vivo expansion of HSC.

PMID:
12935903
[PubMed - indexed for MEDLINE]
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