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Biochem Biophys Res Commun. 2003 Aug 29;308(3):474-9.

Inflammatory mediators expressed in human islets of Langerhans: implications for islet transplantation.

Author information

1
Department of Clinical Immunology, The Rudbeck Laboratory, Uppsala University C11, Sweden. Ulrika.Johansson@klinimm.uu.se

Abstract

Expression of immune modulating mediators in human Islets of Langerhans could have important implications for development of autoimmunity in type 1 diabetes and influence the outcome of clinical islet transplantation. Islets obtained from five donors were analyzed at various times after isolation using cDNA array technology. The Atlas Human Cytokine/Receptor and Hematology/Immunology nylon membranes representing 268 genes and 406, respectively, were used and the relative expression of each gene analyzed. Of the 51 gene products identified, high mRNA expression of MCP-1, MIF, VEGF, and thymosin beta-10 was detected in all islet samples. IL-8, IL-1-beta, IL-5R, and INF-gamma antagonist were expressed in islets cultured for 2 days. IL-2R was expressed in islets cultured for more than 6 days. In conclusion, several inflammatory mediators were expressed in isolated islets, particularly at an early stage after isolation, indicating that a few days of culture could be beneficial for the outcome of islet transplantation.

PMID:
12914774
DOI:
10.1016/s0006-291x(03)01392-5
[Indexed for MEDLINE]

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