Identification of second and third calcitonin receptor-stimulating peptides in porcine brain

Biochem Biophys Res Commun. 2003 Aug 29;308(3):445-51. doi: 10.1016/s0006-291x(03)01413-x.

Abstract

We identified two cDNAs encoding new calcitonin receptor-stimulating peptides (CRSPs) in porcine hypothalamus cDNA library by cross-hybridization with the CRSP cDNA, and designated the second and third peptides as CRSP-2 and CRSP-3. The putative amino acid sequences of prepro-CRSP-2 and prepro-CRSP-3 showed higher identity with that of prepro-CRSP-1 than that of prepro-calcitonin gene-related peptide (CGRP), respectively, and these three CRSPs are considered to form a new family in the CGRP superfamily. RT-PCR analysis demonstrated that both CRSP-2 and CRSP-3 gene transcripts were expressed mainly in the central nervous system and thyroid gland. Synthetic CRSP-2 and CRSP-3 stimulated cAMP production very weakly in LLC-PK(1) cells compared with CRSP and calcitonin (CT). Furthermore, CRSP-2 and CRSP-3 did not elicit a cAMP elevation at all in the COS-7 cells expressing CT receptor or CT-like receptor with or without one of receptor activity-modifying proteins. These results suggest the presence of still unidentified action mechanisms and functions of the peptides in the CGRP superfamily.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism*
  • COS Cells
  • Calcitonin Gene-Related Peptide / classification*
  • Cell Line
  • Cyclic AMP / biosynthesis
  • Molecular Sequence Data
  • Peptides / chemistry*
  • Peptides / genetics
  • Peptides / metabolism*
  • RNA, Messenger / biosynthesis
  • Receptors, Calcitonin / metabolism
  • Receptors, Calcitonin Gene-Related Peptide / metabolism
  • Swine
  • Tissue Distribution

Substances

  • Peptides
  • RNA, Messenger
  • Receptors, Calcitonin
  • Receptors, Calcitonin Gene-Related Peptide
  • Cyclic AMP
  • Calcitonin Gene-Related Peptide

Associated data

  • GENBANK/AB114133
  • GENBANK/AB114134