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Lett Appl Microbiol. 2003;37(3):239-43.

Direct PCR detection of Escherichia coli O157:H7.

Author information

1
Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI, USA.

Abstract

AIMS:

This paper reports a simple, rapid approach for the detection of Shiga toxin (Stx)-producing Escherichia coli (STEC).

METHODS AND RESULTS:

Direct PCR (DPCR) obviates the need for the recovery of cells from the sample or DNA extraction prior to PCR. Primers specific for Stx-encoding genes stx1 and stx2 were used in DPCR for the detection of E. coli O157:H7 added to environmental water samples and milk.

CONCLUSIONS:

PCR reactions containing one cell yielded a DPCR product.

SIGNIFICANCE AND IMPACT OF THE STUDY:

This should provide an improved method to assess contamination of environmental and other samples by STEC and other pathogens.

PMID:
12904226
[Indexed for MEDLINE]
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