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Biotechnol Lett. 2003 Jul;25(13):1007-11.

Resonant Raman quantification of zeaxanthin production from Flavobacterium multivorum.

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  • 1Department of Ophthalmology and Visual Sciences, 50 North Medical Drive, Moran Eye Center, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.


Resonant Raman scattering was used as a novel, rapid, non-destructive optical technique to measure zeaxanthin levels in Flavobacterium multivorum ATCC 55238. Culture broth, after bacterial growth for 40 h, exhibited characteristic resonance Raman vibrational modes at 1159 cm(-1) (C-C stretch) and 1525 cm(-1) (C=C stretch) upon excitation at 488 nm. A striking correlation was observed between the carotenoid level as estimated by HPLC and by resonance Raman spectroscopy.

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