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Nucleic Acids Res. 2003 Aug 1;31(15):e85.

Stable and heritable gene silencing in the malaria vector Anopheles stephensi.

Author information

1
Department of Biological Sciences, Imperial College London, Imperial College Road, London SW7 2AZ, UK.

Abstract

Heritable RNA interference (RNAi), triggered from stably expressed transgenes with an inverted repeat (IR) configuration, is an important tool for reverse genetic studies. Here we report on the development of stable RNAi in Anopheles stephensi mosquitoes, the major vector of human malaria in Asia. Trans genic mosquitoes stably expressing a RNAi transgene, designed to produce intron-spliced double-stranded RNA (dsRNA) targeting the green fluorescent protein EGFP gene, were crossed to an EGFP-expressing target line. EGFP expression was dramatically reduced at both the protein and RNA levels. The levels of gene silencing depended upon the RNAi gene copy number and its site of integration. These results demonstrate that specific RNAi-mediated knockdown of gene function can be achieved with high efficiency in Anopheles . This will be invaluable to systematically unravel the function of Anopheles genes determining the vectorial capacity of the malaria parasite.

PMID:
12888537
PMCID:
PMC169974
DOI:
10.1093/nar/gng085
[Indexed for MEDLINE]
Free PMC Article

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