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J Chromatogr A. 2003 Jun 20;1002(1-2):111-36.

Fungal metabolite screening: database of 474 mycotoxins and fungal metabolites for dereplication by standardised liquid chromatography-UV-mass spectrometry methodology.

Author information

1
Mycology Group, Technical University of Denmark, Building 221, DK-2800 Kgs. Lyngby, Denmark. kfn@biocentrum.dtu.dk

Abstract

A standardised LC-UV-MS micro-scale method for screening of fungal metabolites and mycotoxins in culture extracts is presented. The paper includes data for detection and dereplication of > 400 fungal metabolites to facilitate detection and identification when standards are not available. The data also shows the types of components that can be analysed by positive electrospray (ESI+) mass spectrometry (MS) along with common fragments and adducts of these, as well as giving suggestions on whether UV or ESI+-MS methods should be used. Examples of dereplication of penitrems and macro-cyclic ichothecenes, and detection of several novel compounds are shown. This was done by UV spectroscopy combined with accurate mass determination of adduct and fragment ions obtained by high-resolution orthogonal time-of-flight MS.

PMID:
12885084
DOI:
10.1016/s0021-9673(03)00490-4
[Indexed for MEDLINE]

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