Format

Send to

Choose Destination
Methods Cell Biol. 2003;71:387-416.

Production and use of replication-deficient adenovirus for transgene expression in neurons.

Author information

1
Department of Biochemistry and Molecular Biology, Program in Molecular, Cellular and Integrative Neuroscience, Colorado State University, Fort Collins, Colorado 80523, USA.

Abstract

Adenoviruses infect a wide range of cell types, do not require integration into the host cell genome, and can be produced as replication-deficient viruses capable of expressing transgenes behind any desired promoter. Thus, they are ideal for use in expressing transgenes in the postmitotic neuron. This chapter describes simplifications in the protocols for making recombinant adenoviruses and their use in expressing transgenes in primary neurons of several different types.

PMID:
12884701
DOI:
10.1016/s0091-679x(03)01019-7
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center