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Biotechnol Lett. 2003 Feb;25(3):261-5.

Real-time PCR method to detect Enterococcus faecalis in water.

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U.S. Environmental Protection Agency, National Exposure Research Laboratory, Microbial Exposure Research Branch, 26 W Martin Luther King Dr, Cincinnati, OH 45268, USA.


A 16S rDNA real-time PCR method was developed to detect Enterococcus faecalis in water samples. The dynamic range for cell detection spanned five logs and the detection limit was determined to be 6 cfu/reaction. The assay was capable of detecting E. faecalis cells added to biofilms from a simulator of a water distribution system and in freshwater samples. Nucleic acid extraction was not required, permitting the detection of E. faecalis cells in less than 3 h.

[Indexed for MEDLINE]

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