Format

Send to

Choose Destination
Heart Fail Rev. 2003 Jul;8(3):277-84.

Principles of low temperature cell preservation.

Author information

1
Departments of Bioengineering and Mechanical Engineering, University of California at Berkeley, Berkeley CA 94720, USA. rubinsky@me.berkeley.edu

Abstract

Cell transplantation is becoming an important technique for treatment of heart failure. Preservation is an integral step in any procedure using cells. There are two primary modes of cell preservation at low temperature, hypothermic preservation at temperatures above freezing and cryogenic preservation at temperatures below freezing. Optimal preservation protocols require a fundamental understanding of the principles involved. This review briefly describes the basic mechanisms of damage during hypothermic and cryogenic preservation and the basic principles for developing optimal protocols for preservation of cells.

PMID:
12878837
DOI:
10.1023/a:1024734003814
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center