Send to

Choose Destination
J Med Vet Mycol. 1992;30(6):433-41.

Enhanced phagocytosis and intracellular killing of Candida albicans by GM-CSF-activated human neutrophils.

Author information

Department of Dermatology, University of Glasgow, UK.


Little is known about effector cell modulation regarding phagocytosis and candidacidal activities of human neutrophils in response to granulocyte-macrophage colony stimulating factor (GM-CSF). In neutrophil monolayer assays, pre-treatment of cells with ultrapure GM-CSF at 40 Units ml-1 for a minimum of 1 h showed a significant enhancement of phagocytosis of Candida albicans (phagocytic index of 2.6 compared with 1.6 for unactivated neutrophils) after a 20 min ingestion period (P < 0.001). By counting viable colonies 50% of intracellular yeast cells were killed by non-activated neutrophils following internalization for 2 h. Neutrophils activated for 2 h with 40 Units ml-1 of GM-CSF killed 90% of intracellular blastoconidia (P < 0.02). These results demonstrate that in in vitro assays GM-CSF enhances phagocytosis and intracellular killing of C. albicans.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center