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Curr Opin Mol Ther. 2003 Jun;5(3):235-40.

Analysis of RNA-protein interactions by flow cytometry.

Author information

1
Dana-Farber Cancer Institute, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 1 Jimmy Fund Way, SM 922, Boston, MA 02115, USA. alex_brodsky@dfci.harvard.edu

Abstract

Flow cytometry, in combination with advances in bead coding technologies, is maturing as a powerful high-throughput approach for analyzing molecular interactions. Applications of this technology include antibody assays and single nucleotide polymorphism mapping. This review describes the recent development of a microbead flow cytometric approach to analyze RNA-protein interactions and discusses emerging bead coding strategies that together will allow genome-wide identification of RNA-protein complexes. The microbead flow cytometric approach is flexible and provides new opportunities for functional genomic studies and small-molecule screening.

PMID:
12870432
[Indexed for MEDLINE]

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