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Microbiology. 2003 Jul;149(Pt 7):1871-82.

The Escherichia coli AIDA autotransporter adhesin recognizes an integral membrane glycoprotein as receptor.

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Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung (ZMBE), Westfälische Wilhelms-Universität Münster, Von-Esmarch-Str 56, 48149 Münster, Germany.


The AIDA-I autotransporter adhesin, as a prototype of the AIDA adhesin family, represents a tripartite antigen consisting of the functional adhesin AIDA-I (alpha-domain), which mediates the specific attachment of bacteria to target cells, and a two-domain translocator (AIDA(c)) organized in the beta(1)- and beta(2)-domains. Cellular receptor moieties for the adhesin AIDA-I have not been identified. Here, it is demonstrated that the purified adhesin binds specifically to a high-affinity class of receptors on HeLa cells. Additionally, the adhesin was found to bind to a variety of mammalian cell types, indicating a broad tissue distribution of the receptor moiety. By using complementary techniques, including co-immunoprecipitation and one- and two-dimensional gel electrophoresis, the AIDA-I binding protein on HeLa cells was identified as a surface glycoprotein of about 119 kDa (gp119). The gp119 AIDA-I cellular receptor protein was characterized biochemically and found to be an integral N-glycosylated membrane protein with a pI of 5.2.

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