Format

Send to

Choose Destination
See comment in PubMed Commons below
J Biomol Screen. 2003 Feb;8(1):19-33.

Single-molecule detection technologies in miniaturized high-throughput screening: fluorescence intensity distribution analysis.

Author information

1
GlaxoSmithKline, Direvo, Cologne, Germany.

Abstract

Single-molecule detection technologies are becoming a powerful readout format to support ultra-high-throughput screening. These methods are based on the analysis of fluorescence intensity fluctuations detected from a small confocal volume element. The fluctuating signal contains information about the mass and brightness of the different species in a mixture. The authors demonstrate a number of applications of fluorescence intensity distribution analysis (FIDA), which discriminates molecules by their specific brightness. Examples for assays based on brightness changes induced by quenching/dequenching of fluorescence, fluorescence energy transfer, and multiple-binding stoichiometry are given for important drug targets such as kinases and proteases. FIDA also provides a powerful method to extract correct biological data in the presence of compound fluorescence.

PMID:
12854995
DOI:
10.1177/1087057102239669
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Atypon
    Loading ...
    Support Center