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EMBO J. 2003 Jul 15;22(14):3602-12.

WAVE2 deficiency reveals distinct roles in embryogenesis and Rac-mediated actin-based motility.

Author information

1
Center for Blood Research, 200 Longwood Avenue, Boston, MA 02115, USA.

Abstract

The Wiskott-Aldrich syndrome related protein WAVE2 is implicated in the regulation of actin-cytoskeletal reorganization downstream of the small Rho GTPase, Rac. We inactivated the WAVE2 gene by gene-targeted mutation to examine its role in murine development and in actin assembly. WAVE2-deficient embryos survived until approximately embryonic day 12.5 and displayed growth retardation and certain morphological defects, including malformations of the ventricles in the developing brain. WAVE2-deficient embryonic stem cells displayed normal proliferation, whereas WAVE2-deficient embryonic fibroblasts exhibited severe growth defects, as well as defective cell motility in response to PDGF, lamellipodium formation and Rac-mediated actin polymerization. These results imply a non-redundant role for WAVE2 in murine embryogenesis and a critical role for WAVE2 in actin-based processes downstream of Rac that are essential for cell movement.

PMID:
12853475
PMCID:
PMC165620
DOI:
10.1093/emboj/cdg350
[Indexed for MEDLINE]
Free PMC Article

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