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Protein Sci. 1992 Aug;1(8):1073-7.

Crystallization of proton channel peptides.

Author information

1
Molecular Biology Institute, University of California, Los Angeles 90024-1570.

Abstract

Crystals have been grown of two similar peptides that form ion-conducting channels in diphytanoyl phosphatidylcholine bilayers. These crystals were grown by slow evaporation of the organic solvent, 2,2,2-trifluoroethanol. Crystals of one of the peptides have been characterized by X-ray diffraction, and X-ray data have been measured to 2.3 A resolution. Earlier it was proposed that the ion-conducting channels formed by these peptides consist of four peptides associated as a parallel alpha-helical tetramer. On the basis of the space group and unit cell dimensions of the crystals, a packing scheme for the peptide is proposed that is consistent with a tetrameric channel.

PMID:
1284681
PMCID:
PMC2142170
DOI:
10.1002/pro.5560010812
[Indexed for MEDLINE]
Free PMC Article

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