A simple and rapid method for the preparation of adenosine triphosphatase from submitochondrial particles

R B Beechey; S A Hubbard; P E Linnett; A D Mitchell; E A Munn

doi:10.1042/bj1480533

A simple and rapid method for the preparation of adenosine triphosphatase from submitochondrial particles

Biochem J. 1975 Jun;148(3):533-7. doi: 10.1042/bj1480533.

Authors

R B Beechey, S A Hubbard, P E Linnett, A D Mitchell, E A Munn

PMID: 128353
PMCID: PMC1165573
DOI: 10.1042/bj1480533

Abstract

An almost pure form of the bovine heart mitochondrial adenosine triphosphatase (ATPase) is released from the membrane by shaking submitochondrial particles with chloroform. Analyses on polyacrylamide gels and by electron microscopy, and also sensitivity to inhibitors, show that the chloroform-released enzyme is similar to other ATPase preparations from bovine heart mitochondria.

MeSH terms

Adenosine Triphosphatases / antagonists & inhibitors
Adenosine Triphosphatases / isolation & purification*
Animals
Aurovertins / pharmacology
Cattle
Chloroform
Dicyclohexylcarbodiimide / pharmacology
Electrophoresis, Polyacrylamide Gel
In Vitro Techniques
Methods
Microscopy, Electron
Mitochondria, Muscle / enzymology*
Myocardium / enzymology*
Subcellular Fractions / analysis
Ultracentrifugation

Substances

Aurovertins
Dicyclohexylcarbodiimide
Chloroform
Adenosine Triphosphatases