Immune responses dependent on antigen location in recombinant attenuated Salmonella typhimurium vaccines following oral immunization

FEMS Immunol Med Microbiol. 2003 Jul 15;37(2-3):99-104. doi: 10.1016/S0928-8244(03)00063-4.

Abstract

The subcellular location of a recombinant antigen in recombinant attenuated Salmonella vaccines may influence immunogenicity dependent on exposure of the recombinant antigen to cells involved in systemic immune responses. It has been shown that a recombinant attenuated Salmonella vaccine secreting the recombinant Streptococcus pneumoniae PspA (rPspA) antigen specified by pYA3494 induced protective anti-rPspA-specific immune responses (Kang et al. (2002) Infect. Immun. 70, 1739-1749). A recombinant plasmid pYA3496 specifying a His(6)-tagged rPspA (His(6)-rPspA) protein (no apparent signal sequence) caused the rPspA antigen to localize to the cytoplasm of Salmonella. Salmonella vaccines carrying pYA3494 or pYA3496 expressed similar amounts of rPspA. After a single oral immunization in BALB/c mice with 10(9) colony-forming units (CFU) of the recombinant Salmonella vaccines carrying pYA3494 or pYA3496, IgG antibody responses were stimulated to both rPspA and Salmonella lipopolysaccharide (LPS) antigens. The anti-rPspA IgG titer induced by Salmonella carrying pYA3494 (1.9 x 10(7)) was 10(4) times higher than induced by Salmonella carrying pYA3496 (<2.4 x 10(3)).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Administration, Oral
  • Animals
  • Antibodies, Bacterial / blood*
  • Antigens, Bacterial / immunology*
  • Antigens, Bacterial / metabolism
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology
  • Bacterial Proteins / metabolism
  • Female
  • Lipopolysaccharides / immunology
  • Lipopolysaccharides / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Salmonella Vaccines / administration & dosage*
  • Salmonella Vaccines / genetics
  • Salmonella Vaccines / immunology*
  • Salmonella typhimurium / genetics
  • Salmonella typhimurium / immunology*
  • Subcellular Fractions / metabolism*
  • Vaccination
  • Vaccines, Attenuated / administration & dosage
  • Vaccines, Attenuated / genetics
  • Vaccines, Attenuated / immunology
  • Vaccines, Synthetic / administration & dosage
  • Vaccines, Synthetic / genetics
  • Vaccines, Synthetic / immunology

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Proteins
  • Lipopolysaccharides
  • Salmonella Vaccines
  • Vaccines, Attenuated
  • Vaccines, Synthetic
  • pneumococcal surface protein A