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Hybrid Hybridomics. 2003 Apr;22(2):87-90.

Epitope analysis of the MAb 1AD9 antibody detection site in human protein kinase CK2alpha-subunit.

Author information

1
Medizinische Biochemie und Molekularbiologie der Universitaet des Saarlandes, 66421 Homburg, Germany.

Abstract

1AD9 is a murine monoclonal antibody (MAb) that was produced against the recombinant human alpha-subunit of protein kinase CK2, a pleiotropic and constitutively active serine/threonine protein kinase. We have further characterized this antibody, which is suitable for Western blot, immunoprecipitation and enzyme-linked immunosorbant assay tests. Using an overlapping peptide library, we have identified the epitope targeted by MAb1AD9 characterized by the following sequence: (319)MEHPYF(324).

PMID:
12831533
DOI:
10.1089/153685903321948003
[Indexed for MEDLINE]

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