High-speed simultaneous ion-exclusion/cation-exchange chromatography of anions and cations on a weakly acidic cation-exchange resin column

J Chromatogr A. 2003 May 16;997(1-2):219-24. doi: 10.1016/s0021-9673(03)00631-9.

Abstract

The simultaneous ion-exclusion/cation-exchange separation column packed with a polymethacrylate-based weakly acidic cation-exchange resin of 3 microm particle size was used to achieve the simultaneous high-speed separation of anions and cations (Cl(-), NO3(-), SO4(2-), Na(+), K(+), NH4(+), Ca(2+) and Mg(2+)) commonly found in environmental samples. The high-speed simultaneous separation is based on a combination of the ion-exclusion mechanism for the anions and the cation-exchange mechanism for cations. The complete separation of the anions and cations was achieved in 5 min by elution with 15 mM tartaric acid-2.5 mM 18-crown-6 at a flow-rate of 1.5 ml/min. Detection limits at S/N=3 ranged from 0.36 to 0.68 microM for anions and 0.63-0.99 microM for cations. This method has been applied to the simultaneous determination of anions and cations in several environmental waters with satisfactory results.

Publication types

  • Comparative Study

MeSH terms

  • Anions / isolation & purification*
  • Calibration
  • Cation Exchange Resins*
  • Cations / isolation & purification*
  • Chromatography, Ion Exchange / methods*
  • Crown Ethers
  • Environmental Monitoring
  • Hydrogen-Ion Concentration
  • Quality Control
  • Reproducibility of Results
  • Tartrates
  • Water / analysis

Substances

  • Anions
  • Cation Exchange Resins
  • Cations
  • Crown Ethers
  • Tartrates
  • Water
  • 18-crown-6
  • tartaric acid