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Nucleic Acids Res. 2003 Jul 1;31(13):3688-91.

NEBcutter: A program to cleave DNA with restriction enzymes.

Author information

1
New England Biolabs, Inc., 32 Tozer Road, Beverly, MA 01915, USA.

Abstract

NEBcutter, version 1.0, is a program available via a web server (http://tools.neb.com/NEBcutter) that will accept an input DNA sequence and produce a comprehensive report of the restriction enzymes that will cleave the sequence. It produces a variety of outputs including restriction enzyme maps, theoretical digests and links into the restriction enzyme database, REBASE (http://www.neb.com/rebase). Importantly, its table of recognition sites is updated daily from REBASE and it marks all sites that are potentially affected by DNA methylation (Dam, Dcm, etc.). Many options exist to choose the enzymes used for digestion, including all known specificities, subsets of those that are commercially available or sets of enzymes that produce compatible termini.

PMID:
12824395
PMCID:
PMC168933
DOI:
10.1093/nar/gkg526
[Indexed for MEDLINE]
Free PMC Article

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