Format

Send to

Choose Destination
Antimicrob Agents Chemother. 2003 Jul;47(7):2366-9.

Farnesol biosynthesis in Candida albicans: cellular response to sterol inhibition by zaragozic acid B.

Author information

1
School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588-0666, USA.

Abstract

The dimorphic fungus Candida albicans produces farnesol as a quorum-sensing molecule that regulates cellular morphology. The biosynthetic origin of farnesol has been resolved by treating these cells with zaragozic acid B, a potent inhibitor of squalene synthase in the sterol biosynthetic pathway. Treatment with zaragozic acid B leads to an eightfold increase in the amount of farnesol produced by C. albicans. Furthermore, C. albicans cell extracts contain enzymatic activity to convert [(3)H]farnesyl pyrophosphate to [(3)H]farnesol. Many common antifungal antibiotics (e.g., zaragozic acids, azoles, and allylamines) target steps in sterol biosynthesis. We suggest that the fungicidal activity of zaragozic acid derives in large part from the accumulation of farnesol that accompanies the inhibition of sterol biosynthesis.

PMID:
12821501
PMCID:
PMC161837
DOI:
10.1128/aac.47.7.2366-2369.2003
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center