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PCR Methods Appl. 1992 Feb;1(3):160-3.

A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide.

Author information

1
Biology Division, Beckman Research Institute, City of Hope, Duarte, CA 91010.

Abstract

We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.

PMID:
1282066
DOI:
10.1101/gr.1.3.160
[Indexed for MEDLINE]
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