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Anticancer Res. 2003 Mar-Apr;23(2B):1229-34.

The colon mitosis-inhibitor pyroglutamyl-histidyl-glycine alters expression of immediate-early cancer-related genes in HT-29 cells.

Author information

1
Institute of Pediatric Research, University of Oslo, National Hospital, N-0027 Oslo, Norway. w.h.reichelt@klinmed.uio.no

Abstract

BACKGROUND:

The intestinal mitosis-inhibiting peptide pyroglu-His-GlyOH (pEHG), inhibits normal intestinal epithelial cells and the human colon adenocarcinoma cell line HT-29 and increases the expression of c-fos (1). In this study, we investigated the mechanisms of the growth-inhibiting effects of pEHG.

MATERIALS AND METHODS:

cDNA expression array was hybridized with cDNA from HT-29 cells exposed to pEHG or control. The results were confirmed with Northern blot or real-time PCR.

RESULTS:

pEHG(1 nM) provoked a significant increase in the expression of the early growth response protein 1 (egr-1) after an incubation of 20 minutes, while c-fos was confirmed up-regulated by the same treatment. We further studied the expression of fosB, c-jun and junB, in the AP-1 complex. fosB was up-regulated 20-fold, but only minor effects on jun variants were observed.

CONCLUSION:

pEHG stimulates the gene expression of some immediate-early transcription factors involved in cell proliferation.

PMID:
12820376
[Indexed for MEDLINE]

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