Format

Send to

Choose Destination
BMC Infect Dis. 2003 Jun 19;3:12.

Bioelectronic DNA detection of human papillomaviruses using eSensor: a model system for detection of multiple pathogens.

Author information

1
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. svernon@cdc.gov

Abstract

BACKGROUND:

We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor) in identifying multiple pathogens.

METHODS:

Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes.

RESULTS:

Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples.

CONCLUSIONS:

This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.

PMID:
12814521
PMCID:
PMC165582
DOI:
10.1186/1471-2334-3-12
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center