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ScientificWorldJournal. 2002 May 16;2:1347-50.

Fractionation of hepatic nonparenchymal cells.

Author information

1
School of Biomolecular Sciences, Liverpool John Moores University. john@jgrescon.fsbusiness.co.uk

Abstract

The majority of parenchymal cells from mammalian liver cells can be removed by very low speed centrifugation (50 g) but a simple low-density barrier (1.096 g/ml) is required to remove the remaining parenchymal cells from the 50-g supernatant which contains all of the lower density nonparenchymal cells. Continuous gradients of Nycodenz can provide satisfactory resolution of Kupffer, stellate, and endothelial cells on an analytical basis but the separation of different cell types is not sufficient preparatively. Flotation through a low-density iodixanol barrier can, however, provide a satisfactory enrichment of the least dense nonparenchymal cell--the stellate cells.

PMID:
12805918
PMCID:
PMC6009261
DOI:
10.1100/tsw.2002.283
[Indexed for MEDLINE]
Free PMC Article

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