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FEBS Lett. 2003 Jun 19;545(2-3):151-4.

The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.

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  • 1Institute of Molecular Biology, Department of Molecular Cell Biology, University of Copenhagen, Oester Farimagsgade 2A, Copenhagen, Denmark.


The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.

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